How to Choose ELISA Plates
ELISA plate is widely used in the laboratory, and there are many kinds of it when choosing ELISA Plates. People may be confused about how to choose a suitable ELISA plate. Now let’s share the selection skills of the ELISA plate.
Choosing from ELISA Plates Model
1. First of all, you can choose from some characteristics in appearance, such as non-detachable and detachable ELISA plate models.
- A non-detachable plate is a whole fixed plate with all wells and strips connected together. If some of these wells are used, the remaining unused wells are also inconvenient to use in the next experiment because there may be a risk of contamination.
- Most of the common ones are easy-to-use detachable plates. Detachable ELISA plates have separate strips that are available in both 12-well strips and 8-well strips. And its frame is reusable. If you bought some detachable ELISA plates before, you can just buy some plate strips later.
2. The number of wells is also part of the selection. This 96-well ELISA plate accounts for the majority of the market. It is also the most commonly used one. And 48 wells are still available.
3. It also comes in different colors for people to choose from. It is available in clear, black, and white.
- Transparent ELISA plates are widely used and can be used in common ELISA experiments. It cannot perform luminescence detection like black and white plates, but it can be used for colorimetric experiments.
- Black color has light absorption, and black ELISA plate is generally used to detect strong light experiments such as fluorescence detection. White ELISA plates are often used for general chemiluminescence experiments, like the luminescence experiments.
4. Another is to look at the shape of the bottom of the well. According to the different bottoms, it can be divided into three types: flat bottom, U-shaped bottom, and V-shaped bottom.
- The flat bottom has a relatively low refractive index and is suitable for reading on a microplate reader.
- The ELISA plate with U-shaped bottom has a higher refractive index, which is convenient for operations such as sample addition, sample aspiration, and mixing.
- The shape of the V-bottom plate makes it easy to precisely draw and store samples.
Choosing from ELISA Plates Performance
1. The ELISA plate is generally made of PS material. Due to its own chemical properties, it may be dissolved by some organic solvents during use, and it is easily corroded in contact with strong acids and alkalis. It may also change color after UV exposure. These require attention during operation and should be avoided as much as possible.
2. According to the protein binding capacity of the ELISA plate, it can be divided into high binding capacity, medium binding capacity, and amination.
- After the surface of the high-binding ELISA plate is treated, its protein binding ability is greatly enhanced. Using this plate can relatively reduce the concentration and amount of coated protein and improve the sensitivity. However, it is more prone to non-specific reactions.
- The medium-binding ELISA plate is suitable as a solid phase carrier for macromolecular proteins. Since this type of plate only binds to macromolecules, it is very suitable for use as a solid-phase carrier for unpurified antibodies or antigens. The medium-binding ELISA plate greatly reduces potential non-specific cross-reactions.
- Aminated ELISA plates are suitable as solid-phase carriers for small molecular proteins. Using a suitable buffer, its surface can bind negatively charged small molecules via ionic bonds. Due to the reduction of hydrophobicity during the processing of this type of plate, some protein molecules may not be bound in the experiment.
The above are some aspects that should be paid attention to when choosing ELISA plates, I hope these contents are helpful. Our company also produces ELISA plates, we are full of confidence in our products, you can also contact us for consultation if you need it!