How to Use A Serological Pipette?
About how to use a serological pipette, firstly we should know about the serological pipette. The serological pipette transfers, dispense or aspirate the samples for laboratory use. Serological pipettes usually have graduated markings on the wall to visually measure the amount of liquid aspirated or dispensed. They are top-grade pipettes suitable for aspirating, mixing, and transferring specific volumes of liquids, which also have very stable chemical features. From its name, it can be seen that it is often used in serum-related experimental operations. But what is a serological pipette used for specifically, we will introduce it below.
Serological Pipette Applications
Serological pipettes can mix cell suspensions gently and effectively. They are mixing chemical solutions and reagents can be offered when using a serological pipette as well. They are mainly used to measure and transfer liquids in small volumes with accuracy. Serological pipettes can also be used to move colonies of cells for expansion or more analysis in experiments after the treatment of experimental cell cultures. Careful layering of reagents to create density gradients can be used to their advantage. They are often applied in urinalysis, immunology, hematology, etc.
How To Use Serological Pipette
Serological pipettes are widely used in many fields. It is necessary to know the correct operation of the serological pipette, the following are the steps:
The preparation before use: You should first determine the appropriate volume, then take the product of the right size, open the package from the top, tear only half of the package from the top, and use the wall of the tube to find the correct placement to connect to the dispenser to see if it fits the pipette you will be using. Finally remove the rest of the wrapper to observe the inside, the accuracy level, and the scale line. As it needs to make sure that there is no pollution and no impurities, and the original situation of the serological pipette will be in your mind.
Next, remove the cap of the liquid you want to aspirate, and you can officially operate the suction.
Pinch the upper end of the pipette with your right thumb and middle finger, keep the pipette vertical and insert the lower mouth of the pipette into the liquid to be aspirated. Then gently press the top to slowly aspirate the liquid. Observe the scale markings on the side of the pipette to determine whether the pipette volume is sufficient.
You should be careful when using a serological pipette. Without touching the tip anywhere else now, carefully place the serological pipette close to the target container and dispense the liquid into it. If the container holding the solution is a conical flask, the conical flask should be inclined at 30°, the serological pipette should be kept vertical, and the lower end of the tube should be close to the inner wall of the container. Finally, be sure to dispose of the pipette properly after you are finished transferring the liquid.
Now that you know how to operate serological pipettes, we would like to recommend Hawach serological pipettes to you. Hawach has 6 size varieties to choose from, which are 1mL, 2mL, 5mL, 10mL, 25mL, and 50mL. And double-sided scale mark is easy to observe and record data. The USP grade PS in pure virgin material made, a quality built-in filter, and different colors to mark different scale lines make the Hawach serological pipette popular. And for the tips, there are stretched tips and welded tips for different options. They are compatible with most pipettes on the market.
So you know what is a serological pipette used for and how to use it now. A serological pipette can be a useful aid for transferring solutions in the laboratory if you can handle it with the proper operation when using a serological pipette. They can be applied in bacteriology, cell culture clinical, scientific research, and other fields for small-volume liquid measurement and transfer.