Is It Necessary to Agitate the ELISA Plate?
Before we talk about agitating 96 well ELISA plate, let’s get to know about ELISA plates. There are two types of ELISA plates based on the number of wells: 48-well plates, and 96-well ELISA plates. In general, 96-well ELISA plates are commonly used. The ELISA plate, like its name, stands for the plate used for ELISA experiments. ELISA is an acronym. Its full name is enzyme-linked immunosorbent assay. It is an immunoassay typically used to quantify the level of a specific target in a sample. ELISA plates are used to capture the purity, concentration, and ratio of antigens, antibodies, labeled antibodies, or antigens involved in capturing immunological reactions, and can be used with samples such as saliva, serum, plasma, cell culture supernatants, tissue lysates, etc.
It has a wide range of applications in the laboratory, and one inevitably encounters doubts about practical applications. There are many people who are curious about the purpose of the agitation ELISA plate. If you go to the website and look it up, you might get the idea that the purpose of the agitation of the ELISA plate is to increase the binding rate and that the enhanced signal can be seen together with the shaking.
It has been argued that agitation is necessary while performing ELISA experiments. Shaking, they say, mixes the solution well and increases the chance of effective collisions in the solid phase. Also, it is critical to shake vigorously before reading in order to distribute the product evenly in the well after the substrate is added. It is believed that the agitating facilitates the proper mixing of molecules by causing efficient collisions between molecules in the wells of a 96-well ELISA. This will help complete the reaction in each incubation step. In addition, it was felt that the ELISA 96 well plate was strongly shaken when the substrate was added so that the product was evenly distributed across all wells in the plate prior to taking the absorbance reading. There are also many who believe that shaking improves sensitivity and shortens incubation times.
But there are others who do not think so. They believe there will be an increased risk of cross-contamination between neighboring wells. It has been suggested that shaking enhances antigen-antibody interactions, but no positive results have been obtained. It was also said that improved signals could be seen, but they did not make much difference. They argue that this neither improves the quality of the results nor is practical with agitating well ELISA.
Opinions differ as to what the purpose of the agitation ELISA plate is. So it’s crucial for you to do some research on your experiment before you actually go and operate it yourself. You can check the empirical direction or empirical determination of your predecessors to make sure you are doing the right procedure.
That’s all about agitating 96 well ELISA plate. The smooth progress of experiments requires high-quality product support. We provide the 96-well ELISA you need here. Hawach ELISA 96 well is made of medical-grade PS material, which ensures stability and high binding property. All our products are produced after a rigorous production process for the laboratories. We provide excellent products and pre-sales and after-sales services. If you need 96 well ELISA strip plates or any lab consumables, choose Hawach.