Selection of PCR Consumables
PCR detection techniques have played an indispensable role in nucleic acid detection. After fighting the COVID-19 pandemic for so long, everyone must be familiar with this detection. PCR is a fundamental experimental method in biochemical laboratories, which can be implemented for a large number of DNA fragments with certain procedures. PCR detection techniques can help medical tests save time and make fast and accurate judgments.
At present, most of the COVID-19 detection reagents approved in China use fluorescent PCR methods to detect the virus. The fluorescent PCR method refers to chemicals that add fluorescent groups during the operation of the PCR test technology. With the PCR amplification process, the virus fragments will also accumulate, and a fluorescent signal will be emitted, and the PCR test will produce a positive result. Its experimental detection is crucial, and the choice of consumables has a great impact on the accuracy of the experiment. Here are the options you need to keep an eye on when choosing consumables:
PCR Consumables Material:
PCR consumables are generally PP material. PCR technology consumables are usually in direct contact with samples, while the surface is not easy to adhere to biomolecules, and has good chemical stability, and is not easy to react with samples. Also, the material can withstand temperature changes during autoclaving and thermal cycling. It is the best choice for PCR technology consumables. Medical-grade raw materials can further ensure a better quality of manufactured consumables. Hawach’s PCR consumables are all high-quality medical PP.
Types of PCR Consumables:
There are common types of PCR tubes and PCR plates. When there are not many sample groups, PCR tubes are the best choice. Single tubes and strip tubes are the two common types. The single tube is more flexible to use and comes with a cover for easy operation. Strip tubes are compatible with multichannel pipettes. PCR plates with multiple wells can be used for large-group experiments. Common ones are 96-well and 384-well.
Single tube: 0.2mL, strip tube:0.1mL and 0.2mL, 96 well plate: 0.1mL and 0.2mL, 384 well plate: 40uL.
Skirt in PCR Detection Techniques:
Non-skirted plates are suitable for most PCR instruments but are not stable during pipetting and are not suitable for automation. The semi-skirted plate is suitable for labeling and automation applications and has good pipetting stability. The full-skirt plate is highly stable, and it’s ideal for automated laboratory applications, and can also be adapted to labels.
PCR plates with alphanumeric markers help identify sample locations and facilitate the observation and recording of experiments. PCR plates with raised rims around wells can help seal with sealing film to prevent evaporation and contamination risk. And the ultra-thin tube wall can optimize heat transfer and reduce cycle time. Hawach’s PCR tubes and PCR plates are available for all of the above options.
The above are some of the things you should pay attention to when choosing PCR consumables. We hope you can choose the right consumables. High-quality consumables will help you with your PCR detection techniques. Our company’s PCR consumables can meet your needs in many of the above aspects, and we hope to have the opportunity to carry out long-term cooperation with you.