96 Well Cell Culture and ELISA Plate
When it comes to 96-well plates for cell culture and ELISA, do you have doubts in your mind? Both of these two plates are 96-well, and they look similar in appearance, but they have different names for different experiments. So what are the differences between them and whether they can be used interchangeably? Do these questions also appear in your mind? These are the questions that will be discussed next.
First of all, let’s briefly introduce these two products, so that the relevant memories of these two products can appear in your mind.
96 Well ELISA Plate:
It is indispensable equipment used in conjunction with a microplate reader for ELISA experiments.
Enzyme-linked immunosorbent assay is to allow specific antigens or antibodies to attach to the surface of the ELISA plate and maintain its immune activity. These antigens or antibodies will be linked with a certain enzyme to form enzyme-labeled antigens or antibodies, which retain both their immune activity and enzymatic activity.
During the measurement, the tested antibody or antigen and enzyme-labeled antigen or antibody are reacted with the antigen or antibody on the surface of the ELISA plate according to different steps. The method of washing is used to separate the antigen-antibody complex formed on the ELISA plate from other substances, and finally, the amount of enzyme bound to the ELISA plate will be proportional to the amount of the tested substance in the sample.
After adding the substrate for the enzymatic reaction, the substrate is catalyzed by the enzyme into a colored product, and the amount of the product is directly related to the amount of the tested substance in the specimen. It can be qualitatively or quantitatively analyzed according to the shade of color reaction.
The ELISA plate is the most basic tool of the whole experiment, and the experiment cannot be carried out without it.
96 Well Cell Culture Plate:
A cell culture plate is a biological tool. Cell culture plates are used to grow cells in the wells. People can select 6 well, 12 well, 24 well, 48 well, and the commonly used 96 well cell culture plates. The choice can be determined according to the type of cultured cells, the required culture volume, and different experimental purposes.
It has different bottom shapes for customers to choose from according to their needs. Flat-bottom plates are available for any type of cell, so most experiments use flat-bottom plates. U and V-shaped plates are only used when there are some special requirements. The round bottom is usually used for the analysis or preservation of samples. The round bottom is better to absorb the liquid and is not easy to retain.
ELISA and Cell Culture Plate Differences:
a. Different appearances: Elisa has black, white, transparent, and printing marks options, as well as detachable types. While the cell culture dish is usually transparent and non-removable, the structure is simple.
b. Different uses: ELISA plates are not used for cell culture and are mainly used for protein detection after ELISA reaction. The cell culture plate is mainly used to culture cells, and can also be used to measure protein concentration.
c. Different requirements: Elisa requires higher production requirements and specific enzyme labeling working solutions. The cell culture plate can be used according to normal production standards.
d. Different prices: Production and price are closely related, and ELISA plates are generally more expensive than cell culture plates.
Those are all the differences between 96-well plates for cell culture and ELISA. Although they all have 96 wells, there are actually quite different. If you are interested in buying them, you can come and see the Hawach cell culture plate and 96 well ELISA strip plates. They won’t disappoint you.
