What is Differential Centrifugation?
The collection, preparation and separation of many samples in the experiment require the use of a centrifuge for centrifugation. The application of centrifugation is becoming more and more extensive. Have you mastered the method of sample centrifugation? Today we will introduce to you the centrifuge centrifugation methods: differential centrifugation. And we hope that you can have a better understanding of it.
Differential Centrifugation Definition
Centrifugation is a method of separating substances at different rotational speeds according to their densities. Differential centrifugation is a very common centrifugation method. The differential centrifugation method uses the difference in the sedimentation of different particles in the centrifuge under the condition of alternately using low-speed and high-speed centrifugation, and uses different strengths of centrifugal force to separate mixture particles of different erogenous sizes in layers.
The differential centrifugation method adopts the method of gradually changing the centrifugation speed to separate particles of different densities and structures in the sample. The operation process is generally to firstly centrifuge to let the larger particles settle to the bottom of the tube out, and then the supernatant and separate it from the sediment. The supernatant containing the small particles is then centrifuged at high speed to separate the second fraction of the precipitate. In this way, the separation is repeated many times to achieve the separation of the required substances step by step.
This method is suitable for the separation of various substances in mixed samples with large differences. Differential centrifugation can only perform approximate material separation and is often used to extract crude products before other separation methods. Various particles with similar densities and masses are not easy to separate by this method.
Advantages of Differential Centrifugation
- This method can be used for the initial separation of a large number of samples, can undertake a large processing capacity, and has relatively high processing efficiency.
- The differential centrifugation method is simple to operate. It only needs to repeat centrifugation to separate the sediment to extract the supernatant, and then continue to extract the supernatant after re-centrifugation. It does not require too many strict skills, and the error rate is low.
- The separation time is short during differential centrifugation, and the cost of time spent is low. It is very suitable for operation in the spare time, and the sample can be processed conveniently and quickly.
- This method has high repeatability and can be repeated many times. The number of separations is controlled by oneself, and the separation task can be completed freely according to the experimental requirements.
- Differential centrifugation can be used for simple pretreatment, which is convenient for subsequent experiments and provides convenience for formal experiments.
- Differential centrifugation has a wide range of applications and can be used to separate mitochondria, chloroplasts, proteins, and some liquid samples that require supernatants.
This is all about what is differential centrifugation. We mainly introduce the differential centrifugation definition and the advantages of differential centrifugation. There are also many other centrifugation methods in the experiment, which can be selected according to requirements under different needs. If you need to buy centrifuge tubes and centrifuge bottles for your centrifugation experiments, Hawach hopes to provide you with the best quality products. Looking forward to your information, we look forward to meeting more long-term partners.